Viral Vector Process Development

Plasmid production

In the context of viral vector production, plasmids can be adapted to contain the instructions for producing viruses with specific genomes. Through standard plasmid cloning, viruses can therefore be engineered to contain important genes for either gene therapy or vaccination purposes. Viral vectors can be used as a powerful tool for the delivery of this genetic information into mammalian cells. During the plasmid production stage, plasmids are either constructed in the laboratory or selected from existing stock. The type and number of plasmids you require will depend on the viral vector you choose to use and whether you intend to use transient or stable cell lines. Learn more about plasmid production in our webinar!

Bioprocessing for AAV vector production

Cells will need to be harvested and the viral vectors isolated for characterization and quantification. An important part of this process is determining the ratio of viral vectors with genomes to those without, known as the full/empty ratio. This can be done using methods including ELISA, electron microscopy, analytical ultracentrifugation (AUC), and high-pressure liquid chromatography (HPLC), each of which has benefits and limitations of its own. For information on AAV vector production workflow in a bioreactor, check out this application note!